Purification, structure features and anti-atherosclerosis activity of a Laminaria japonica polysaccharide》.pdf

International Journal of Biological Macromolecules 81 (2015) 926–935 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j o u r na l h o me p a g e : w w w. elsev /locate/ijbiomac Purification, structure features and anti-atherosclerosis activity of a Laminaria japonica polysaccharide Fu-Hua Peng, Xue-Qiang Zha ∗ , Shao-Hua Cui, Muhammad-Naeem Asghar, Li-Hua Pan, Jun-Hui Wang, Jian-Ping Luo ∗ School of Biotechnology and Food Engineering, Hefei University of Technology, PR China a r t i c l e i n f o a b s t r a c t Article history: A homogeneous polysaccharide (LJP12) was isolated from Laminaria japonica by diethylaminoethyl- Received 18 June 2015 cellulose and Sephacryl S-500 chromatography, with a molecular weight of 2.31 × 106 Da. Monosac- Received in revised form 12 August 2015 charide analysis showed that LJP12 was mainly composed of arabinose, xylose, mannose, glucose and Accepted 17 September 2015 galactose in a molar ratio of 1:0.17:1.54:2.64:0.18. For these monosaccharides, mannose was suggested Available online 21 September 2015 to be 1,4-linked and 1,3,6-linked while glucose was linked by 1,6-glycosidic bond. The xylose, arabinose and galactose were suggested to be the terminal residues. To study the effects of LJP12 on protecting Keywords: −/− against athe