Quantitation of ultraviolet-induced single-strand breaks using oligonucleotide chip》.pdf

a n a l y t i c a c h i m i c a a c t a 6 2 2 ( 2 0 0 8 ) 195–200 a v a i la b le at ww w.scie ncedirect. com j o u rn al h omepage: www.elsevi er.c om /locate/aca Quantitation of ultraviolet-induced single-strand breaks using oligonucleotide chip Sukdeb Pal a a b c , Min Jung Kim , Jaebum Choo , Seong Ho Kang , Kyeong-Hee Lee d, Joon Myong Song a,∗ a Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151-742, South Korea b Department of Applied Chemistry, Hanyang University, Sa-1-dong 127, Ansan, Kyunggi-do 426-791, South Korea c Department of Chemistry and Basic Science Research Institute, Chonbuk National University, Jeonju 561-756, South Korea d Applied Imaging Research Group, Korea Electrotechnology Research Institute, Gyeonggi-Technopark, 1271-11, Ansan 426-901, South Korea a r t i c l e i n f o a b s t r a c t Article history: A simple, accurate and robust methodology was established for the direct quantifi- Received 28 March 2008 cation of ultraviolet (UV)-induced single-strand break (SSB) using oligonucleotide chip. Received in revised form Oligonucleotide chips were fabricated by covalently anchoring the fluorescent-labeled 24 May 2008 ssDNAs onto silicon dioxide chip surfaces. Assuming that the possibility of more than Accepted 27 May 2008 one UV-induced SSB to be generated in a small oligonucleotide is extremely low, SSB Published on line 4 June 2008