Rapid NOS-1-derived nitric oxide and peroxynitrite formation act as signaling agents for inducible NOS-2 expression in vascular smooth muscle cells》.pdf

Pharmacological Research 100 (2015) 73–84 Contents lists available at ScienceDirect Pharmacological Research j o u rn al h omepage: w w w. elsev /locate/yphrs Rapid NOS-1-derived nitric oxide and peroxynitrite formation act as signaling agents for inducible NOS-2 expression in vascular smooth muscle cells Karin Scheschowitsch a , João Alfredo de Moraes b , Regina Sordi a,1, Christina Barja-Fidalgo b , Jamil Assreuy a,∗ a Department of Pharmacology, Universidade Federal de Santa Catarina, Santa Catarina, Brazil b Department of Cell Biology, Universidade do Estado do Rio deJaneiro, Rio deJaneiro, Brazil a r t i c l e i n f o a b s t r a c t Article history: Septic vascular dysfunction is characterized by hypotension and hyporeactivity to vasoconstrictors and Received 1 May 2015 nitric oxide (NO), reactive oxygen species and peroxynitrite have a prominent role in this condition. Received in revised form 4 July 2015 However, the mechanism whereby the vascular dysfunction is initiated is poorly understood. Based on Accepted 1 August 2015 previous studies of our group and the literature,we hypothesize that constitutive nitric oxide synthases Available online 4 August 2015 (c-NOS) and peroxynitrite may play a role in the development of septic vascular dysfunction. Bacterial lipopolysaccharide (LPS) and interferon- (IFN) were used to stimulate rat aorta smooth muscle c