Rapid repair of DNA double strand breaks in Arabidopsis thaliana is dependent on proteins involved in chromosome structure maintenance》.pdf

DNA Repair 8 (2009) 413–419 Contents lists available at ScienceDirect DNA Repair j o u rn al h omepage: w w w. elsev /locate/dnarepair Brief report Rapid repair of DNA double strand breaks in Arabidopsis thaliana is dependent on proteins involved in chromosome structure maintenance Jaroslav Kozak a , Christopher E. West b , Charles White c , José A. da Costa-Nunes d , Karel J. Angelis a,∗ a Institute of Experimental Botany AS CR, Na Karlovce 1, 160 00 Praha 6, Czech Republic b CPS, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK c Génétique, Reproduction et Développement, UMR CNRS 6247 - Clermont Université - INSERM U931, 63177 Aubière cedex, France d ISA, Universidade Técnica de Lisboa, 1349-017 Lisboa, Portugal a r t i c l e i n f o a b s t r a c t Article history: DNA double strand breaks (DSBs) are one of the most cytotoxic forms of DNA damage and must be repaired Received 18 July 2008 by recombination, predominantly via non-homologous joining of DNA ends (NHEJ) in higher eukaryotes. Received in revised form 5 November 2008 However, analysis of DSB repair kinetics of plant NHEJ mutants atlig4-4 and atku80 with the neutral comet Accepted 9 November 2008 assay shows that alternative DSB repair pathways are active. Surprisingly, these kinetic measurements Available online 31 December 2008 show that DSB repair was faster in the NHEJ mutant lines than in wild-type Arabidopsis . Here we p