Reactive dyes and textile effluent decolorization by a mediator system of salt-tolerant laccase from Peniophora cinerea》.pdf

Separation and Purification Technology 135 (2014) 183–189 Contents lists available at ScienceDirect Separation and Purification Technology journal homepage: /locate/seppur Reactive dyes and textile effluent decolorization by a mediator system of salt-tolerant laccase from Peniophora cinerea Sérgio Moreira a, Adriane M.F. Milagres a, Solange I. Mussatto b,⇑ a Department of Biotechnology, Engineering College of Lorena, University of São Paulo, Estrada Municipal do Campinho s/n, 12602-810 Lorena, SP, Brazil b Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands a r t i c l e i n f o a b s t r a c t Article history: Peniophora cinerea is a white-rot fungus with ability to decolorize dyes in saline textile effluent. After cul- Received 17 October 2013 tivation in a medium composed by sucrose, corn steep liquor, copper, and other minor components, P. Received in revised form 3 August 2014 cinerea produced 1000 U/L of laccase, but no lignin peroxidase or manganese peroxidase. The produced Accepted 4 August 2014 laccase was purified by precipitation with ammonium sulfate (80%) and anion exchange chromatography. Available online 23 August 2014 Isoelectric focusing revealed a total of eight salt tolerant laccase isoenzymes with pI between 3 and 6, and molecular weights in the range of 26.2 and 72.6 kDa. Five compounds were then tested and compared for Keywords: their ability