Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer》.pdf
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Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer》.pdf
Vaccine 33 (2015) 4807–4812
Contents lists available at ScienceDirect
Vaccine
j o u r n a l h o me p a ge : w w w. elsev /locate/vaccine
Recombinant invasive Lactococcus lactis can transfer DNA vaccines
either directly to dendritic cells or across an epithelial cell monolayer
Marcela de Azevedo a , Marjolein Meijerink b , Nico Taverne b , Vanessa Bastos Pereira a ,
Jean Guy LeBlanc c , Vasco Azevedo a , Anderson Miyoshi a , Philippe Langella d,e ,
Jerry M. Wells b , Jean-Marc Chatel d,e,∗
a Laboratório de Genética Celular e Molecular, ICB, UFMG, Belo Horizonte MG, Brazil
b Host Microbe Interactomics, Wageningen University, Wageningen, The Netherlands
c CERELA-CONICET, San Miguel de Tucumán, Argentina
d INRA, UMR1319 Micalis, Jouy-en-Josas, France
e AgroParisTech, UMR Micalis, F-78350 Jouy-en-Josas, France
a r t i c l e i n f o a b s t r a c t
Article history: Lactococcus lactis (L. lactis), a generally regarded as safe (GRAS) bacterium has recently been investi-
Received 23 March 2015 gated as a mucosal delivery vehicle for DNA vaccines. Because of its GRAS status, L. lactis represents
Received in revised form 30 June 2015 an attractive alternative to attenuated pathogens. Previous studies showed that eukaryotic expression
Accepted 24 July 2015
plasmids could be delivered into intestinal epithelial cells (IECs) by L. lactis, or recombinant invasive
Available online 1 August 2015
strains of L. lactis, lea
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