响应面法优化辣木叶总黄酮超声波辅助提取工艺.pdf

热带农业科技 2015,38 （4） -22- Tropical Agricultural Science Technology 响应面法优化辣木叶总黄酮超声波辅助提取工艺 * 王苗苗，赵有为，赵开丽，万 力，刘凤霞 ，王可兴，潘思轶 （华中农业大学食品科学技术学院，武汉430070） 摘要摘要：：采用超声波辅助提取辣木叶总黄酮，并利用响应面法对提取工艺参数进行优化。首先通过单 因素试验分别考察超声波提取的料液比、提取时间、提取温度和提取功率对辣木叶总黄酮提取率的 影响，确定各因素的适宜水平；在此基础上进行四因素三水平的Box-Behnken 中心组合设计法设计响 应面试验，分析不同因素、不同水平对辣木叶总黄酮得率的影响。超声辅助提取辣木叶总黄酮的最 佳提取工艺条件为：料液比1∶35（g/mL）、提取时间40 min、提取温度52 ℃、功率210 W，在此条件下 辣木叶总黄酮提取率的预测值为5.082%，验证试验所得总黄酮含量为5.17%，所得回归模型拟合情况 良好。 关键词关键词：辣木；总黄酮；超声波提取；响应面分析 中图分类号：S59 文献标识码：A 文章编号：1672-450X（2015）04-0022-07 —————————————— OptimizationOptimization ofof Ultrasonic-Ultrasonic- AssistedAssisted ExtractionExtraction ProcessProcess forfor TotalTotal FlavonoidsFlavonoids fromfrom MMoorriinngg aa oolleeiiff-- eerraa LeavesLeaves byby ResponseResponse SurfaceSurface MethodologyMethodology * WANG Miaomiao, ZHAO Youwei, ZHAO Kaili, WAN Li, LIU Fengxia , WANG Kexing, PAN Siyi Instituteof FoodScience andTechnology of Huazhong University ofAgriculture,Wuhan430070,China Abstract:Abstract: Response surface methodology （RSM ）was used to optimize the experimental conditions for ultrasonic-assisted ex- traction of total flavonoids from Moring a oleifera leaves. First, the effect of solid-to-liquid ratio, extraction time, extraction temperature, and extraction power on the extraction yield of total flavonoids were investigated using single factor experiment. Based on the results, the experimental conditions were optimized using a four-variable, three-level Box-Behnken experimental design, and the optimum conditions for the extraction were obtained as follows: 1:35 （g/mL）for the solid-to-liquid ratio, 40 min for the extraction time, 52 ℃ for the extractio