脂联素对钙化大鼠血管组织的影响.pdfVIP

808 武鳖医堂!Q!!生!旦箜堑鲞笠§塑丛笪』g!i!旦垒里：!尘：堑：堕!：!：垒!鲤生垫!! 脂联素对钙化大鼠血管组织的影响 闰 亮1，王 昆2 【摘要】 用。方法大鼠随机分为正常对照组、钙化组和脂联素组。制备大鼠血管钙化模型。用ELISA法测定血浆和血管组织中脂 联素的含量，用real—time R1的mRNA水平，用Westernblot方法检测组织中受体 PCR方法检测组织中脂联素与其受体adipo adipoR1蛋白含量，测定血管中钙含量及碱性磷酸酶活性。结果与对照组相比，钙化大鼠血浆和血管组织中脂联素的含量 R1 明显降低，分别降低了47．1％和57．1％；钙化组大鼠血管组织中APN和adipomRNA水平较对照组有显著降低，分别降低 了65．0％和72．0％；钙化大鼠的血管组织中adipo 调，APN可明显抑制钙化大鼠血管组织中钙含量和碱性磷酸酶的活性，提示APN可抑制维生素域和尼古丁引起的血管钙化。 【关键词】血管钙化；脂联素；脂联素受体 【中国图书分类号】 R54 Influenceof onvascularcalcificationinrats adiponectin YAN andWANGKun2．1．MedicalExamination of of Center，302nd Lian91 HospitalPLA，Beijing Medical of Service，GeneralPLA，Beijing100853，China Hospital Toobservethe of andits inVasCular theroleofadi- 【Abstract】objectivechangesadiponectinreceptors calcification，and oncalcifiedtissues．MethodsMaleSDratswereinducedvascularcalcificationvitaminD3and orwithout ponectin by nicotine，with andalkaline levelsofAPNin andvasculesweremeasured adiponectin．Calciumphosphatase(ALP)wereassayed．The plasma by ELISA，ThemRNAlevelsofAPNand R1weredetected real—time levelof R1 wasdetected adipo byusing PCR，the adipoprotein by Western withcontrol APNconcentrationsin andcalcifiedbloodvesselsdecreased blotting．ResultsCompared group，the plasma by 47．1％and mRNAlevelsofAPNand R1decreased65，0％and72．0％．The R1 de· 57．1％．respectively．The adipo by adipoprotein creased foldincreasecalciumand83．4％increaseinALP wereobservedincalcifiedbloo