复方鹰不泊对环磷酰胺免疫抑制小鼠血清IFN-γ、IL-2及血T淋巴细胞亚群影响的研究.pdfVIP

EffectsofFuFangYingBuPoonBloodIFN-yandIL-2andBlood The-dependentT-LymphocytesofCyclosphamide-immunosuppr- EssantMice Postgraduate:HuSong Tutor:ZhangYuLiang.HeHao HunanCollegeofTCM,Changsha_China.410007 ABSTRACT Objective:TostudytheeffectsofFuFangYingBuPoonbloodIFN-yand IL-2andbloodthe-depedentT-lymphocytesofCyclophosphamideimmu- nnosuppressantmice Methods:ThemodelofCyclophosphamide-immunosuppressantmicewere establishedandwererandomlydividedinto3groupsbyhome.Thenormal controlgroupwastreatedwithNS,andtheothergroupsweretreatedwith NS、FuFangYingBuPo、polysacc-haridilentinusedodestrespectivelyfor 14days.IFN-YandIL-2inbloodserumandthenumberofCD3+,CD4+and CD8+cellsofbloodthy-dependentT-lymphocytesinCyclophosphamide- immunosuppressantmicewereobservedbythetechniquesofELISAand SABCrespectively. Results: IFN一丫andIL-2inbloodserum inthegrouptreatedwith FuFangYingBuPowashigherthanthatinthemodelgroup,difference betweentwogroupswassignificant(p0.05，p0.005);Thenumberof CD3+.CD4+.CD8+cellsofbloodthy-dependentT-lymphocytesgrouptreated withFuFangYingBuPowashigherthanthatinthemodelgroup,difference betweentwogroupswassignificant(p0.005) Conclusion:FuFangYingBuPocanenhanceIFN-YandIL-2inbloodserum ofCyclophosphamide-immunosuppressantmice,andimprovethenumber ofCD3+、CD4+、CD8+cellsofbloodthy-dependentT-lymphocytesin Cyclophosphamide-immunosuppressantmice. words:YingBuPo Cyclophosphamide immunosuppression IFN- yIL-2 Bloodthy-dependentT-lymphocytes 前 言 自1989年Choo等川发现丙型肝炎病毒(tlcv)基因组以来，有关HCV 感染已引起广泛关注。HCV是威胁人类健康的重要病毒，其所引起的丙 型肝炎严重地危害人民健康，全世界约有 1亿7千万人感染licv，至少 有 70%发展成慢性丙型肝炎，在 10-20年内可进展成肝硬化，且与原 发性肝癌发生密切相关川。 在丙型肝炎发病机制方面，免疚机制最为重要 ”‘‘免疫应答包括体液 免疫和细胞免疫，通常FICV因其高度的变异性能有效地逃避体液免疫 ”‘， 而特异性细胞免疫应答(尤其是细胞毒性淋巴细胞-CTL)在清除病毒及被 病毒感染的细胞中起重要作用，但是机体细胞免疫反应并未执行其正常 功能，故flcv感染后易形成持续感染状态从而漫性化 ”‘。首先CTL的数 量及功能不足以清除病毒 3‘卜另有研究表明CHC(慢性丙