九叶青花椒遗传多样性的形态和分子鉴定.pdfVIP

西北植物学报, 2008, 28( 10) : 2103- 2109 Act a Bot . Bor eal . 2Occident . Sin. * : 100024025(2008)07 邓洪平, 徐 洁, 陈 锋, 宋琴芝 ( 西南大学 生命科学学院 三峡库区植物生态与资源实验室, 重庆 400715) : 以九叶青花椒 研究材料, 以花椒和竹叶花椒 研究对照, 采用 Anderson 设计的/ 杂种指数0 、/ 形象化散 点图0 等形态鉴定方法, 结合RAPD 分子标记技术及聚类分析等手段, 从表观形态到分子水平对其遗传多样性来 源、现状进行了分析和研究。结果表明: ( 1)九叶青花椒是花椒和竹叶花椒长期渗入杂交, 经多年的世代积累形成 的具有复杂遗传多样性的杂交复合体。(2)在九叶青花椒杂交复合体形成过程中, 竹叶花椒对其遗传多样性的贡 献大于花椒。( 3) 九叶青花椒已具有相对独立且稳定的形态特征, 形成了不同于花椒及竹叶花椒的分类群, 将其定 义 变种是科学可行的。研究结果 该资源的开发与利用提供了理论与实践依据。 :九叶青花椒; 遗传多样性;渗入杂交; 杂交复合体;RAPD :Q789 :A Morphological and Molecular Identification on Genetic Diversity of Zanthoxy lum ar matum var. n ovemf olius DENG Hon 2pin , XU Jie, CHEN Fen , SONG Qin2zhi ( School of Life Science, Lab oratory of Plant E colo y and Resources in th e Three Gor e Reservoir Re ion, Southwest University, Chon qin 400715, Ch ina) Abstract: Methods of morpholo ical and molecular wer e used to study on the or i in and status of enetic di2 versity of Zanthoxylum armatum var. novemf olius, with two controllin exper iment, Z. armatum and Z. planisp ium. The r esults showed that : ( 1) Z. armatum var. novemf olius was from many enerations accu2 mulation of intro ressive hybridization between Z. armatum and Z. planisp ium, and it had become a hybr id complex contained many populations with complicated enetic diversities; (2) Z. armatum contributed more enetic diversities to the hybrid complex than Z.planispium; ( 3)That Z. armatum var. novemf olius was a variety of Z. armatum could be affirmed by its own unattached and steady morpholo ic characters distin2 uished fr om those of Z. armatum and Z.planisp ium. The results had