温敏型pnipam荧光探针的设计与制备.pdfVIP

摘要 摘要 发剂、溶剂类型对ATRP反应的影响。 ATRP反应的特征。 和荧光光谱等方法对FL．PNIPAAm荧光探针的结构和性能进行了表征。相关测试表明， 的低。 进而选用无毒且低廉的9．氨基吖啶(9．AA)为原料，与溴乙酰溴在四氢呋喃(THF) 发剂。 荧光分光光度计对所9．AA．PNIPAAm的荧光响应性进行了表征，发现其在碱性条件下， 荧光强度随着pH值的增加而增大，且随着碱性的增加，其发射峰发生红移，说明该双 臂型9-AA—PNIPAAm中带有荧光基团，有较好的荧光响应性。 关键词：N．异丙基丙烯酰胺，原子转移自由基聚合，荧光探针，pH．／温度敏感性 Abstract Abstract Inthis aCIorBrend was group radical atomtransfer synthesizedby polymerization(ATRP)using (ECP)or or N。N．Nf。NⅢ．Ntpentamethyldiethylenetriamine(PMDETA)or indifferentsolvents ethyl]amine(Me6TREN)鹊ligand andmixed effects sulfoxide(DMSO)，water of andsolventonArRPreactionwerediscussed．Structureand catalyst．1igand．initiatortypes meansof molecularofthePNIPAAmwerecharacterized nuclear number-averageweight by magneticresonance(1H-NMR)andgelpermeationchromatography(GPC)． ItWaSfoundthatthemolecular ofthe PNIPAAmWaSlOWin distribution weight resulting at40℃under condition．Thelowcritical 2-propanol n[ECP]：n[CuCl]：n[Me6TREN】=1：1：2 solution PNIPIAAmWaSdramaticdecreaSewith temperature(LEST)of increasing molecular number-averageweight． nle fluorescenceWas obtained polymeric probe successfullythroughnucleophilic with in was tobe substituteofPNIPAAm．Clfluore