《Morphological and Hormonal Identification of Porcine Atretic Follicles and Relationship Analysis of Hormone Receptor Levels During Granulosa Cell Apoptosis In vivo.》.pdf
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《Morphological and Hormonal Identification of Porcine Atretic Follicles and Relationship Analysis of Hormone Receptor Levels During Granulosa Cell Apoptosis In vivo.》.pdf
Availableonlineatwww.sciencedirect.corrt
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MorphologicalandHormonalIdentificationofPorcineAtreticFolliclesand
RelationshipAnalysisofHormoneReceptorLevelsDuring Granulosa
CellApoptosisInvivo
YU De-bing,YU M in.1i,LIN Fei,JIANG Bao—chun,YANG Li—na,WANG Si—yu,ZHAO Ying and
WNAG Zheng—chao
DepartmentofAnimalGenetics,BreedingandReproduction,CollegeofAnimalScienceandTechnology,NanjingAgriculturalUniversity
Nanjing210095,P.R.China
Abstract
Recentrepotshavedemonstratedthatfollicularatresiaisinitiatedorcausedbygranulosacellapoptosisfollowedbytheca
celldegenerationinmammalianovaries,butthemechanism offollicularatresiaisstilltobeelucidated.Therefore,ourpresent
studywasdesignedto examineourhypothesisthatthechangesoffollicularmicroenvironmentinducethegranulosacell
apoptosisduringpocrinefollicularatresiainvivo.W efirstlyisolatedintactporcineantralfolliclesandidentifiedthem into
threegroups,healthyfollicles(HF),earlyatreticfollicles(EAF)andprogressedatreticfollicles(PAF)throughmorphology
andhistology.Tofurtherconfimatheirstauts,wedetectedhormonelevelsinfollicularfluidsandtheexpressionlevelof
apoptosisgeneBaxinrganulosacells.Therateofprogesterone(P)andestradiol(E2)wasincreasedwithhteexpression
ofBax,indicatinghomr onecanbeusedasamarkerofgranulosacellapoptosisorfollicularatresia.Finally,weanalyzed
theexpressionlevelofhormonereceptorgenesingranulosacellsandtheirrelationshipwithfollicularatresia.InPAF.the
expressionofProgesteronereceptor(PGR)wasincreasedsignificantlywhileestradiolreceptor(ER)hadnonotablechanges,
whichsuggestingtheincreased-PGRacceleratedtheeffectofP-stimulatedgranulosacellapoptosis.Thedramaticincreasing
ofandrogenreceptor )expressioninPAFandtheobviousincreaseofutmornecrosisfa
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