《大鼠卵巢组织低温保存后形态学及超微结构改变（英文）》.pdfVIP

Chinese-German Journal of Clinical Oncology September 2012, Vol. 11, No. 9, P527–P532 DOI 10.1007/s10330-012-1026-5 Morphological and ultrastructural changes in rat ovarian tissues after cryopreservation Rumana Jafarey, Jing Yang Reproductive Medical Centre, Renmin Hospital of Wuhan University, Wuhan 430060, China Received: 26 May 2012 / Revised: 20 June 2012 / Accepted: 9 July 2012 © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2012 Abstract Objective: The aim of the study was to observe and compare the effects of cryopreservation and thawing meth- ods on rat ovarian tissues. Methods: Twenty 5–6 weeks old SPF-SD female rats were randomly divided into two groups, with ten rats in each group. Freshly isolated ovaries saved as a control (group 1: fresh ovaries) in formalin-fixed or vitrified immediately after dissection (group 2: vitrified ovaries). Ovaries in vitrified group were processed into thin slices then cryo- preserved, stored in liquid nitrogen for 21 days, rapidly thawed and grossly examined. All of the collected ovaries underwent hematoxylin and eosin-stained paraffin serial sections and observed the microscopic evaluation in vitrified ovaries. Results: Grossly the vitrified ovaries turned pale color and the size was same as before freeze. The vitrified ovarian tissue had normal anatomical structures of cortex and medulla under the microscope and had no difference with the fresh control ovarian tis- sue. The number and distribution of the follicles were similar with the fresh ovarian tissue, but had smaller size and the gap between oocyte and the surrounding granulosa cells was increased. Few ooctyes were in irregular appearance howeve