《5-FCCD 系统对荷大肠癌裸鼠肿瘤的杀伤效应》.pdfVIP

世界华人消化杂志990605 世界华人消化杂志 WORLD CHINESE JOURNAL OF DIGESTOLOGY 1999年 第7卷　第6期 Vol.7　No.6 1999 5-FC/CD 系统对荷大肠癌裸鼠肿瘤的杀伤效应* 崔　龙　林言箴　朱正纲　陈雪华　顾琴龙　刘炳亚　郁宝铭 摘　要 目的　研究cd基因对大肠肿瘤的特异杀伤作用，探索自杀基因靶向治疗大肠癌的有效 途径. 方法　逆转录病毒感染法将G1ceacdNa及pcd2分别转导入高分泌CEA的大肠癌细胞Lovo 中，再分别接种到裸鼠皮下. 成瘤后腹腔给予5-FC(500mg/kg)治疗，观察肿瘤重量的变 化及病理学特点. 7 8 及2.1×10 结果　含G1ceacdNa及pcd2 逆转录病毒载体的病毒滴度分别为：1.3×10 CFU/L. 所有转基因成功并接种动物均成瘤. 腹腔给予5-FC治疗后发现，转由CEA基因 顺式转录调控序列(TRS)驱动cd基因的组织特异性重组逆转录病毒载体G1ceacdNa的肿 瘤对5-FC的敏感性明显高于转非cea调控cd基因的肿瘤，治疗结束后肿瘤重量分别为 31mg±8mg及113mg±23mg(P＜0.01). 结论　本实验提示cea转录调控序列可控制cd基因在CEA阳性的大肠癌组织中高效表 达，进而在前药5-FC 的作用下发挥选择性杀伤肿瘤的作用. 主题词　结直肠肿瘤；cd基因；癌胚抗原；基因治疗 中国图书馆分类号　R735.34 Killing effect of 5-FC/CD system on nude mice bearing human colorectal carcinoma* CUI Long, LIN Yan-Zhen, ZHU Zheng-Gang, CHEN Xue-Hua, GU Qin-Long, LIU Bing- Ya and YU Bao-Ming Shanghai Institute of Digetive Surgery, Department of Surgery of Ruijin Hospital, Shanghai 200025, China Subject headings　colorectal neoplasms; cd gene; carcinoembryonic antigen; gene therapy Abstract AIM　To study the killing effect of cytosine deaminase (cd) and targeted therapy for colorectal. METHODS　The recombinant retroviral vector G1 ceacdNa, in which carcinoembryonic antigen (CEA) promoter was employed to drive the cd gene, was constructed. After packaging in PA317 cells and havesting viral supernant, the retroviral construct and pcd2 were produced to CEA highly produced clolorectal 万方数据 file:///E|/qk/hrxhzz/hrxh99/hrxh9906/990605.htm（第 1／6 页）2010-3-22 23:35:41 世界华人消化杂志990605 6 carcinoma cell line Lovo and then 1.2×10 parental Lovo cells, Lovo cells transfected pcd2 retroviral vect