吡格列酮减轻糖尿病大鼠肾脏损伤及其对肾组织PCX表达影响.pdf

目 录 中英文缩写对照表·············································································· ··················· 3 中文摘要 ····························································································· ··················· 5 英文摘要 ····························································································· ··················· 8 1 引言 ································································································· ··················· 12 2 材料与方法······················································································ ··················· 13 2.1 材料 ······························································································ ··················· 13 2.2 方法 ······························································································ ··················· 15 2.2.1 糖尿病大鼠模型的制备及分组 ················································ ··················· 15 2.2.2 尿液标本采集············································································ ··················· 16 2.2.3 尿白蛋白测定············································································ ··················· 16 2.2.4 尿肌酐测定················································································ ··················· 18 2.2.5 尿沉渣PCX测定 ········································································ ··················· 19 2.2.6 血样采集及组织取材································································ ··················· 20 2.2.7 肾脏组织病理形态变化···························································· ··················· 21 2.2.8 免疫组化法检测肾组织PCX蛋白的表达································· ··················· 22 2.2.9 RT-PCR检测大鼠肾皮质中PCX mRNA 的表达····················· ··················· 23 2.3 统计学分析················································································· ··················· 29 3 结果 ······································································