《5株鸡传染性支气管炎病毒M基因克隆及序列分析》.pdfVIP

福建农欺学报23(4)：342--345，2008 Journal Sciences FujJan ofAgricultural S株鸡传染性支气管炎病毒M基因克隆及净列分析 施少华1，陈 珍1，邴国霞2，熟 祥2，刘金华2，黄瑜1 (1．福建省农业科学院畜牧酱医研究所，福建福州 350013；2．中国农业大学动物医学院，北京100094) 克隆到pGEM—TEasy载体t，获得M蔟阂的蕈缎质粒。序列分析表明，5株IBV分离株闯的M蒺因核苷敬溺 离株分詹3个群；分离毒株M蛋自的氆一螺旋结梅生要分布在N端翦100个氮基酸残越的肽段区，其他的部分妻 要是8一折叠和无规鲻卷益结梅，表明IBV分离撩M蛋自在蕉鞭序列秘二缀结构上穗辩保守。 关键词：鸡传染饿支气管炎病毒；M基因；序列分析 858．31 中图分类号：S855．3：S 文献标识码：A and ofM offiveinfectiousbronchitisviruses Cloninganalysisgene SHIShao-hua’，CHENZhenl，BINGGuo-xia2，LIU Yul Xian92，LIUJing—hua2，HUANG (1．Animal and Institute。F酊ian HusbandryVeterinary AcademyofAgriculturalSciences，Fuzhou，Fujian 350013tChina；2 100094，China) CollegeofVeterinaryMedicine·ChinaAgriculturalUniversity，Beijing entirecDNAoftheM offiveinfectiousbronchitis meansofRT Abstract：The gene viruses(IBVs)wereamplifiedby wereclonedinto vectors．Thenucleotide showed89．O％一100％ —PCR．They pGEM—TEasy sequenceanalysis the5isolates，and as tothereferencecontrol。Basedonthe homologyamong 85。7％一99。3％homologycompared 5 werein oftheaminoacids，theseisolates 3 a-helix werefound phylogeneticanalysis separategroups．Theregions inthefirst100aminoacidsoftheIBVs’M remainderwas8-sheetsandcoils．Thus，the mainly proteins．The prima— to and structuresofthelBVs’M be conservative． ry secondary geneappearedrelatively bronchitis words：infectiousvirus；Mgene；phytogenetieanalysis Key 鸡传染性支气管