《HepG2 cells recovered from apoptosis show altered drug responses and invasiveness》.pdfVIP

Liver HepG2 altered drug responses and invasiveness after recovery Original Article/ HepG2 cells recovered from apoptosis show altered drug responses and invasiveness Shan-Shan Wang, Xin Xie, Chung Sing Timothy Wong, Pui Ying Choi and Ming Chiu Fung Hong Kong, China BACKGROUND:  Cancer relapse, associated with increased KEY WORDS:  cancer recurrence; drug resistance and rate of metastasis, often follows completion apoptosis; of chemotherapy but the cancer escape mechanisms are still recovery; incompletely understood. Percutaneous ethanol injection (PEI) liver cancer; has been used for treating hepatocellular carcinoma (HCC) percutaneous ethanol injection; for decades, while the recurrence after PEI treatment remains 5-fluorouracil a major limitation. Recent evidence mounted that cancer cells could survive from chemical induced apoptosis, suggesting a potential route through which cancer relapse may occur. This Introduction study focuses on the consequence of HepG2 recovery from ethanol-induced apoptotic event. ancers often respond well to chemotherapy METHODS:  The model of HepG2 recovery from ethanol- Cinitially but end up with cancer recurrence induced apoptotic event was established by live cell imaging, which remains to be a major cause of cancer BrdU assay and Western blotting. MTT assay, wound healing deaths. Common features of recurrent cancers include assay and invasion assay were used to investigate the behavior of increased mobility, stronger drug resistance, and quicker HepG2 after recovery.