《HT29细胞黏着斑激酶表达与其对5-氟尿嘧啶敏感性的关系研究》.pdfVIP

· 堡夔垩医芏塑查；!塑生!旦箜!!鲞蔓!塑丛笪』鱼h也￡垦垒y!!；i盟里!△P堕!；!塑 ·421 H129细胞黏着斑激酶表达与其对争氟尿嘧啶敏感性的关系研究 陈玉英杨黎潘凤梁后杰 5-氟尿嘧啶(5-FU)敏感性的变化。方法利用针对FAK基因的特异性寡核苷酸序列及对照序列构建重组体，酶切并测序鉴定，用脂 提供了可能。 【关键词】黏着斑激酶；RNA干扰；结肠肿瘤；基因表达 【中国图书资料分类号1R34-33；R735．35 betweenthe offocal钡lllesi蚰ldn嬲eandthe ofHI抄cellsto5-fluorouradl Relafionship expression sensitivity aL of Medical Chen Li，Pan Military Yuying。YangFeng，etDepartmentOncology，SouthwestHospital，nlird 400038，China Uuniversity，Chongqing RNA constructedtoinhibitthefocaladhesion interference(RN加)expressionvector啪s 【Al】st惴妇Objective in the to determined．MethodsOne coloncarcinomaHT29cells。andthenthe of cells5-tluorouraeil(5_兀J)was s10e- pression sensitivity dfic of short andits control were and basedonFAKcDNAse— pairoligonudeotides、Il，ithhairpin negative sequencedesignedsynthesized into voctor therecombinant identificationtherestrictionendonu- wereinserted to by quences，thenthey pGenesil-1 generate plasmids．After transfectedHT29cells TM2000．nestabletransfected desseandDNA recombinantwere into sequencing．theplasmids bylipofectamine mediuln inFAK inHT29cellsbeforeandafterRNAinterference cellswereselectedina G418．Thechange expression containinggeneticin was and