《Kvβ1.3亚基显著影响DPO-1对表达在卵母细胞上的Kvl.5通道的阻断作用》.pdfVIP

·离子通道和一I)律失常专栏· * 细胞上的Kvl．5通道的阻断作用 赵宁1 杜以梅1 夏程琨2 高明1 邹安若1 廖玉华1 [摘要] 阻断作用的影响。方法：在非洲爪蟾卵母细胞上异源表达克隆Kvl．5及KvBl．3通道基因，用双电极电压钳技术 论：Kv81．3亚基显著抑制DPO-1对表达在卵母细胞上的Kvl．5通道的阻断作用，但不改变其电压、频率及浓度 依赖性。可能机制是KvSl．3亚基与DPO-I相互竞争Kvl．5孔区内部的某些结合位点。 [关键词]Kvl31．3亚基；DP(J_1；卵母细胞；Kvl．5通道 [中图分类号]R541．7[文献标志码]A [文章编号]1001—1439(2010)03—0171—04 subunitaffectsblockofKvl．5 channel Kv]il．3accessory potassium DPO-1in by Xenopusoocytessignificantly 2 D己，YimeilXjA ZoUAnru01LJAOYuhual ZHAONin91 ChengkunGAOMin91 (1ResearchCenterofIon of ofCardiovas- Channelopathy，DepartmentCardiology。2Department cular ofScienceand Surgery，UnionHospital，HuazhongUniversity Technology，Wuhan， 430022，China) YimeiandLIA0 author：DU Corresponding Yuhua，Email：liaoyh27@163．corn Abstract theeffectsofKvBl．3subnuitonblockofKvl．5 channel Objective：Toinvestigate potassiumby DPO-1in andKvBl．3cRNAchannelwere in Xenopusoocytes．Method：Kvl．5 gene expressedXenopusoocytes andchannelcurrentswererecordedstandardtwo-microelectrode incontrolcondi— using voltageclamptechniques tionorDP(F1intervention．Result：DP(klinhibited channelina Kvl．5+KvBl．3coexpressing voltage-，frequency- and manner．Theblock ofKvl．5 channelwasdiminished concentration—dependentefficacy potassium significantly inthe of wasincreased