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《EGCG Enhances TRAIL-mediated Apoptosis in Human Melanoma A375 Cell Line》.pdf
UnivSci
JHuazhongTechnol[MedSci]
29(6):771-775,2∞9
DOt10.1007/s11596.009—0620-4
771
EGCGEnhancesTRAIL·-mediatedinHumanMelanoma
Apoptosis
A375CellLine
QinSHEN(沈琴)’,FenTIAN(田芬)+,PingJIANG(蒋萍)’,YanqiuLI(李艳秋),LiZHANG(张丽),JingjingLU(卢静静),
Jiawen
LI(李家文)4
China
necrosisfactor-related a
Summary:Tumor apoptosis.inducingligand(TRAIL)ispromising
of
anti.cancer a constituenttea.In
agent.Epigallocatechin.3.gallate(EGCG)ispolyphenolic green
ofEGCGandTRAILonhumanmelanomaA375cells
this e饪-cctofcombineduse
study,inhibitory
wasexaminedandthe mechanism cellsweredividedinto4
possible investigated.The groups:control
EGCG+TRAIL
group,EGCGgroup(EGCG:10,20txg/mL),TRAILgroup(TRAIL:25n∥mE)and
inhibitionwasmeasuredintheA375cellstreatedwithdifierent
group(combinedgroup).Thegrowth
concentrationsof MTT wasas-
TRAIL“25,50,75,100,125,150ng/mL)byassay.Theapoptosis
ofDR4andDR5weredetectedflow and
sessedflowcytometry.The cytometry
by expressions by
western activitiesof and werede
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