《Livin基因沉默对大肠癌细胞凋亡和化疗敏感性的影响》.pdfVIP

芒塑笠些巫!墨固真塞堑盔墅擅堡塑塑立塑毡痘筮璺丝煎墅喧墓丝越 ·3287· Livin基因沉默对大肠癌细胞凋亡和化疗敏感性的影响 严 红 彭淑梅 罗其泰邱春雷 延卫东 (井冈山大学医学院内科教研室，江西 吉安 343000) 目的 siRNA (摘要] 观察沉默Livin基因对大肠癌HT-29细胞凋亡和化疗敏感性的影响。方法采用脂质体转染技术将化学合成的Livin 细胞的生长，促其凋亡．提高5一FU的化疗敏感性。Livin基因有望成为大肠癌治疗的新靶点。 (关键词)Livin；大肠癌；凋亡抑制蛋白；BNA干扰；细胞凋亡；化疗敏感性 【中图分类号】耵35．3 (文献标识码】A 【文章编号】 1005-9202(2010J22-3287-04 Effects ofLivin on and ofcolorectal曲tl-dnomcells genesilencingchemotherapeuticsensitivityapoptosis YAN Shu-Met．LUOaL Hong，PENG Qi-Tai。et ofInner Medicine，Medical Department College，JinggangshanUniversity，Ji’all343000，Jiangxi，China To theeffectsofLivin on and ofcolorectalcar- Objective 【Abstract】 explore genesilencingchemotherapeuticsensitivityapoptosis was cinomaHT．29cells．MethodsLivin siRNA were and transfectedinto specific oligonucleotidesdesignedsynthesizedartificially．SiRNA eolorectalcarcinomaHT-29cells wasdetectedWesternblotandRT—PCRTbetransfect． bylipofectiontechnology．Transfectioneffieiency by edanduntransfectedcellswereincubatedinthe of5．FU．Cellular activetieswere MrITr．The of presence pmliferation assayedby apoptosis cellswas flow withthoseofothertransfectedandcontrol levels assayedby cytometry(FCM)．ResultsCompared groups group，theprotein of LivinandthemRNA ofLivinotand weredecreased aftertransfected expressions Living significantly by growth inhibitionratesofcellsincubat