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目 录
中文摘要····················································1
英文摘要 ····················································2
引 言 ·····················································4
正 文 ·····················································5
1.实验材料和方法············································5
1.1 实验材料·················································5
1.1.1 实验动物···············································5
1.1.2 药物与试剂·············································5
1.1.3 主要仪器 ···············································5
1.2 实验方法·················································5
1.2.1 脑梗塞动物模型制作法 ···································5
1.2.2模型判断标准 ···········································6
1.2.3 动物分组 ···············································6
1.2.4 操作方法 ···············································6
1.3 取材与观察指标 ···········································7
1.3.1 脑组织的采集和固定 ·····································7
1.3.2脑组织BDNF的检测方法 ···································8
1.3.3脑组织c-Fos的检测方法 ··································8
1.3.4观察指标 ···············································8
1.4 统计学方法 ···············································9
2.实验结果··················································9
2.1 实验动物数量分析·········································9
2.2 大鼠神经功能评定·········································9
2.3 苏木素-伊红染色(HE-染色) ·······························10
2.4三个时间点各组大鼠脑组织BDNF表达比较····················10
2.5三个时间点各组大鼠脑组织c-Fos表达比较···················11
3.讨 论 ···················································12
3.1祖国医学对脑梗塞的认识 ··································12
3.2现代医学对脑梗塞的认识 ··································15
3.3 关于脑梗塞模型的制作和神经功能的评价 ····················18
3.4 浅刺调督综合疗法的理论及依据 ····························18
3.4.1 浅刺针法的理论和依据 ··································18
3.4.2调督法治脑梗塞的理论及依据 ····························20
3.4.3 辅以补阳还伍汤的理论和依据 ····························21
3.5脑梗塞与脑源性神经生长因子BDNF 的关系···········
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