加味生脉饮治疗冠心病临床及实验地研究.pdf

The Clinical and Experimental Research of Jia Wei Sheng Mai Yin on Coronary Heart Disease Speciality: Traditional Chinese and Western medicine combined with clinical Author: Wang Nan Wang Nan Tutor: Prof.Li Rui Prof.Li Rui ABSTRACT Objective ：To study the clinical effect of Jia Wei Sheng Mai Yin (JWSMY) on the patients of coronary, and inquire into the mechanism of experimental atherosclerosis in Rabbits that its Resistance to atherosclerosis. Methods ：1.Clinical research:60 patiens of CHD with stable tired angina were chosen and divided into 2 groups randomly,one group were treated with anti-angina drugs and JWSMY as experiment group ,while the other group were treated with anti-angina drugs as control group.Three months later,we observed the effet on the patiens.2. Experimental Study: 24 New Zealand white rabbits were randomly divided into three groups, n = 8. The －1 － first group were fed a high cholesterol diet; the second group with JWSMY (1g ·d ·Kg 1), at the same time the rabbits were fed a high cholesterol diet; the third group to a normal diet. Three groups were fed four months later,we took some blood from the ear margin veins, and used enzymology methods to determine each animal blood lipid levels.Then we killed animals, took chest aortas, and made HE staing and MASSON staing to observe atherosclerosis and spot mass fiber.Also we used immunity organization chemistry to observe smooth muscle cells protein expression. Results ：1.Clinical research:Compared with control, the treatment group angina illness integral significantly reduced (P0.05) after tr