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附子汤半仿生法实验地研究.pdf

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Exper imental Study on Formula Fuzi Decoction By Semi-Bionic Extraction Specialty：Chinese Meteria Medica Author：Wang Hui Tutor：Lv Qing-tao Abstract Objective : To optimize the best Semi-Bionic Extraction(SBE) condition for Fuzi Decoction and futher verifying the correctness and practicality of Semi-Bionic Extraction(SBE). Method : Using the even designment to optimize the best extraction conditions of Semi-Bionic Extraction for Fuzi Decoction,with paeoniflorin,ginsenoside Rg1,ginsenoside Re,total aconitine,total saccharine,volatile oil,tolal integral calculus area of 50% alcohol solution by HPLC,dried extract weight as the indexs.To optimize the extraction methods of combined ingredients of Fuzi Decoction.According to the former conditions,the five kinds of extract metods,including the semi-bionic extraction(SBE),the water extraction(WE),the semi-bionic extraction by precipitation with alcohol(SBAE), the water extraction by precipitation with alcohol(WAE) and the alcohol extraction,were compared in the contents of the index ingredients and in the fingerprinting of the rabbit，s blood serum. Result: The technological conditions for SBE:the PH value of the water for the 1st,2nd,and 3rd decoctions are in order 6.0,7.5,8.5,and the extraction times are 2h,1h and 0.5h respectively.Mixing Aconitum carmichaeli Debx,Paeonia lactiflora Pall,Rhizoma Atractylodis Macrocephalae,Poria,Radix Ginseng together to extract is the best combinamic.The sequence of total index Y value of five kinds of alcohol precipitation concentration is: Y Y 61.25%＞ 55% Y Y Y .Combined with the production,the best alcohol precipit