Effectsofsalicylicacidandsilicononoxidativedamageandantioxidantactivityinmungbeancuttingsunderborontoxicity.docVIP

Effects of salicylic acid and silicon on oxidative damage and antioxidant activity in mung bean cuttings under boron toxicity. Abbas J. Muhammad Abdullah I. Shaheed Univ. of Babylon, College of Sci, Dept. of Biology. Abstract The effects of silicon (Si) and salicylic acid (SA) on the proline, lipid peroxidation (MDA), protein content, transpiration rate and lipoxygenase activity in mung bean (Phaseolus aureus Roxb.) cuttings under boron (B) toxicity were studied. In general, Boron toxicity at (250- 600) μg/ml significantly reduced growth parameters and morphological symptoms represented by bleaching of primary leaves, in addition to chlorosis and necrosis. The physiological parameters of B toxicity were significantly declined for protein (25 %) and transpiration rate (50 %), but there was significant increase in malondialdehyde (52 %), lipoxygenase activity (68 %) and proline (90 %) compared with control .For alleviating B toxicity, SA and Si were supplied. That resulted in increasing in MDA content, whereas decrease proline and maintaining of lipoxygenase activty. Besides, retention of protein content and transpiration rate. Keywords : salicylic acid. Silicon. boron toxicity. oxidative damage. Antioxidants. Introduction Maintaining adequate level of nutrients including Boron(B) in soil is essential for the normal growth of plants. However, B usually causes toxicity when it is applied in level more than needed for normal growth (Marschner, 1995). Toxicity of B is most commonly found in arid and semi -arid regions (Nable et al., 1997). Boron toxicity in the crop plants occurs because of higher B concentration in irrigation water or higher applications of B fertilizer to correct deficiency or use of compost material containing high B level. It may occur also due to fly ash or soils developed from marine sediments (Nable et al.,1997). Although boron (B) is an essential micronutrient, an excess causes negative physiological effects including: reduced root cell