荧光标记试剂制备和在高效液相色谱-质谱中的应用.pdf

脑、造纸厂废水实际样品的测定。结果表明，衍生反应条件温和，衍生产率 高，该方法的线性范围宽，重现性好，灵敏度高。 神经递质的高效液相色谱。质谱分析。建立了BCEOC用于五种神经递质的荧 光标记液相色谱分析法，实现了氨基酸类神经递质和含有一级胺与二级胺的 单胺类神经递质的同时标记，对大鼠端脑中的神经递质实现了准确测定。采 用二十种氨基酸的BCEOC柱前衍生荧光检测法实现了大鼠血浆、麻花艽、 秦艽中氨基酸的测定。 关键词：高效液相色谱，质谱，荧光标记试剂，柱前衍生，脂肪 酸，脂肪胺，神经递质，氨基酸 Abstract toits liquid po、Ⅳedhl Hi曲perfornlance been aIld in fields entllllsiasticallywidelyadoptedmally sepamtionc印aci吼has 0fsciemmcresearch as and印plication，such scienceand most llsedme也odsin f瓠test a11dthe isalsooneoftlle deveIopiIlg wideIy tobe all aresuitable directly compounds aIlal舛calchemist阱Ho、vevernot ofthe andⅡle HPLCbecauseof也e1imi诅tion a11alyzedby compoundpmpenies contentsofthc low very co玳sponding most still tobeoneofthe sensitive de把ctionis recognized of Tberefore，derivatizationaIlal”es，such弱锄ino caIlalter ∞ids，诵也nuorescencelabelingreageInSh粥been嘶delyadopted．It makethemeasier and of and thecb锄ical ta昭etcompo吼ds physicalpropenies to mis and fiVenoVel analyze．Inp印erW