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Exercise 1: The Microscope Page 4 Letter “e” Observation The letter “e” should appear upside down and backwards. Under high magnification, you will see only part of the“e” and may see dots of ink rather than a continuous flow of ink. Crossed Threads Observation-Describe your observations Under low (4x) power: Two of the threads are almost in focus, but it is impossible to get both completely focused at the same time. By focusing first on 1 thread then on the second, it is possible to judge which thread is on top. This is an example of being able to visualize in 3-D. Under 10x power, you still have some depth perception and can see the threads crossing over each other although you are seeing a smaller area of threads and may need to move the stage to see more of the threads. Under 40x power, you lose all depth perception and are unable to determine which thread is on top and which is on the bottom. You are seeing smaller areas of the thread because of the higher magnification. Under which power was it easiest to get the overlapping threads mostly in focus? Low power Page 6 Study Questions 1. Summarize the function of the following parts of the microscope: Nosepiece: Holds objective leases Iris diaphragm: Constricts or dilates to allow varying amounts of light to pass through the slide Coarse adjustment: Focusing knob, usually the large one, that allows you to focus your slide more quickly. Use with low power only otherwise you may damage the slide or the lens. 2. What does the term “parfocal” mean? The microscope is designed so that when you switch to the higher objective lens, the microscope will automatically be partially focused. You will only need minor adjustments of the fine adjustment knob. 3. Why is it important to start with the lowest power objective lens? This is the magnification under which it is easiest to find your focus quickly using the coarse adjustment knob. At low magnification, your depth of field is best so the precision required for f