仁用杏品种SRAP遗传多样性分析及指纹检索系统的开发.docVIP

园 艺 学 报 2014，41（6）：1191–1197 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 收稿日期：2013–12–27；修回日期：2014–05–05 基金项目：河北省高等学校科学技术研究重点项目（ZH2007116，Z * 通信作者 Author for correspondence（E-mail：apf2002@） 仁用杏品种SRAP遗传多样性分析及指纹检索 系统的开发 艾鹏飞，苏 姗，靳占忠* （河北科技大学生物科学与工程学院，石家庄 050018） 摘 要：利用SRAP标记对24份仁用杏品种进行了遗传多样性分析。15对引物共扩增出280条带， 其中241条为多态性谱带，多态性比率为85.34%；每个引物组合扩增谱带数在13 ~ 24条之间，平均为 18.7条；多态性信息含量（PIC）在0.28 ~ 0.65之间，平均为0.51。基于引物Me4-Em4扩增的多态性谱 带，构建的指纹检索系统可以区分24个仁用杏品种。根据SRAP扩增结果，利用UPGMA法构建树状聚 类图，在相似系数为0.70处可将24个仁用杏品种分为4组，聚类结果与形态分类基本一致。 关键词：仁用杏；SRAP；遗传多样性；指纹检索系统 中图分类号：S 662.2 文献标志码：A 文章编号：0513-353X（2014）06-1191-07 Analysis of Genetic Diversity and Development of Fingerprinting Key Among Accessions of Kernel-using Apricot Using SRAP Markers AI Peng-fei，SU Shan，and JIN Zhan-zhong* （College of Biological Science and Engineering，Hebei University of Science and Technology，Shijiazhuang 050018， China） Abstract：In order to evaluate the genetic relationship among accessions of kernel-using apricot， sequence related amplified polymorphism（SRAP）markers were used to evaluate genetic diversity of 24 samples. Fifteen primer pairs generated 280 bands，241 bands（85.34%）of which were polymorphic ones. SRAP primer pairs amplified 13 to 24 bands with an average of 18.7 bands per primer pair. Polymorphic information content（PIC）ranged from 0.28 to 0.65 with an average of 0.51. A fingerprinting key， established on the base of polymorphic bands amplified by a primer pair of Me4-Em4，could discriminate 24 accessions of kernel-using apricot. A dendrogram was constructed based on SRAP data using UPGMA culster method. The 24 accessions were classified into 4 major groups at the genetic similarity coefficient of 0.70，which was basically corresponded with the genetic relationship based on morphological traits. Key words：kernel-using apricot；S