P.fluorscens发酵生产胞外多糖的结构分析及活性评价.pdfVIP

4)-β-D- Manp -(1, 4)-β-D-Glcp -(1, 4)-β-D-Manp 。 5. 对 P. fluorescens PGM37 胞外多糖的体外吸湿保湿和抗氧化活性进行探 究，该多糖保湿性能远远优于甘油并仅次于透明质酸，在一定的浓度范围内可以 达到水分含量的动态平衡；多糖对·OH、DPPH ·自由基的清除、抗以卵黄脂蛋 白为底物的脂质过氧化均具有显著的效果，且存在良好的量效关系，随着 EPS 浓度的增大，其清除或抑制效果增强，EC50 值分别为 7.44 mg /mL、16.76 mg/mL、 17.18 mg/mL，具有较强的体外抗氧化活性，在化妆品和医药方面有着潜在应用 价值。 P. fluorescens PGM37 胞外多糖和植物魔芋葡甘聚糖（KGM ）结构具有很大 的相似性，而KGM 现已被国内外学者多次证实具有很好的吸湿保湿以及抗氧化、 抗肿瘤活性等，探索实验证明该胞外多糖也具有一定的吸湿保湿和抗氧化生物性 能。目前未见报道有微生物产此种类的葡甘聚糖，当前的葡甘聚糖仅有魔芋这一 单一植物来源。P. fluorescens PGM37 产糖培养基成分要求简单、产量稳定、生 产成本低，适合工厂的规模化生产，有很大开发前景。本论文的研究为微生物来 源的葡甘聚糖的工业化生产、探索结构和活性的关系以及潜在的开发应用提供理 论基础。 关键词：Pseudomonas fluorescens ；胞外多糖；发酵；结构；活性 II Structure analysis and biological activities evaluation of exopolysaccharide produced by P. fluorescens Abstract Polysaccharides have increasing market demand in recent years because of their biological activities. Bacterial extracellular polysaccharides (EPS) come from liquid fermentation of bacteria, the advantages of bacterial fermentation are short incubation period, high yield, stable quality which present a kind of high efficiency, energy saving industrial producing method of polysaccharides. The culture conditions of bacterial strains differ from each other, and the yields of extracellular polysaccharides of directional screened strains could be improved to different levels by optimizing the culture conditions, these extracellular polysaccharides usually have potential biological activities. The exopolysaccharide producing strain in this paper was isolated from beer barley and preserved in our lab, which was found with relatively stable yield and character. The liquid fermentation culture conditions were optimized using the extracellular polysaccharide production as an index. The m