消脂通脉颗粒的研制.pdf

Preparation and Evaluation of the granule of Xiaozhitongmai. Speciality : Pharmacy of TCM Author: Ma Qiuju Tutor: Prof.Yu Weiping o Abstract Objective: The Preparation and Evaluation of the compound Chinese medicine Xiaozhitongmai. Methods: We extracted the volatile oil from Curcuma and Atractylodes macrocephala and Citri reticulatae by the Supercritical CO2 Fluid extraction.Using the yield of volatile oil as index ，we selected the Optimum extraction conditions by the orthogonal test, which were 25MPa(the extracting pressure), 45 ℃(the extracting temperature ) ,2.5hour(the extracting time). Using the ultizision ratio of oil and the oil-beared ratio and the yield of inclusion compound as indexes , we selected the Optimum conditions for inclusion by the orthogonal test ,which were 1:8(w/w) (the ratio of oil β-CD ), 50 ℃(the inclusion temperature),1hour(the inclusion time).To find the best condition of extracting the active constituents of Xiaozhitongmai Granule with alcohol, using the yield of alcohol extractive and content of AlisoB23-acetate as the detective indexes,we selected Optimum extracting conditions by The orthogonal text. Experiments showed best condition was Volume ratio of 1:8(w/w) (the ratio of herbs and 70% alcohol), extracting for 2 times(first times 60min,second times 30 min) and water temperature was 50℃.Extracting solution with alcohol was Concentrated by pressure-reduced distillation and dried in Vacuum dry box. and water-content of alcohol extractive was smaller than 4%. in the Preparation Process，Coix lachrymajobi was used as Cppropriate ,Steviosin was used to correct the taste and 80% alcohol was us