大王仙丹水萃物对重组人类酪胺酸酶活性之抑制.pdfVIP

大王仙丹水萃物 对重组人类酪胺酸酶活性之抑制 江善宗1黄雅琪2 陈俊宏2 1国立台湾海洋大学 2静宜大学化妆品科学系 Inhibitionofrecombinanthumantyrosinase activitybywaterextractsofIxoraduffiicv Shann-TzongJiang1Ya-ChiHuang2Gen-HungChen2 1DepartmentofFoodScience,NationalTaiwanOceanUniversity,No.2Pei-NingRd.,Keelung,Taiwan20224 2DepartmentofCosmeticScience,ProvidenceUniversity,200ChungChiRd.,Salu,Taichung,Taiwan43301 中文摘要；酪胺酸酶(EC1.14.18.1)是一种含铜的多酚氧化酶，黑色素生成的关键酵素，此酵素与动物 的黑色素生成及植物褐变有关。于人类，酪胺酸酶是合成毛发、皮肤及眼睛色素之关键酵素。近年来，很多研 究致力于从植物或微生物中寻找天然酪胺酸酶抑制剂。这些酪胺酸酶抑制剂有很广的用途，尤其是在医药或化 妆品之应用均与其抗色素生成之性质相关。本研究乃利用重组人类酪胺酸酶当探针去分析来自变叶木、鹅掌 藤、矮仙丹及大王仙丹水萃物之抑制重组人类酪胺酸酶活性。其中，大王仙丹水萃物抑制重组人类酪胺酸酶 (IC50:58.38mg/ml)之能力几乎可以媲美曲酸(IC50:58.31mg/ml)。 关键词：重组人类酪胺酸酶;抑制剂;大王仙丹水萃物 中图分类号=C931.2 Abstract: Tyrosinase(EC1.14.18.1),akindofcopper-containingpolyphenoloxidase,isresponsiblefor notonlymelanizationinanimalsbutalsobrowninginplants.Inhuman,tyrosinaseisthekey enzymetosynthesizepigmentformingthecolorofhair,skinandeyes.Inrecentyears,therehas beenanincreasinginterestinfindingnaturaltyrosinaseinhibitorsfromplantsandmicroorganisms. Thetyrosinaseinhibitorsshouldhavebroadapplications,especiallyinpharmaceuticalandcosmetic productsinrelationtoanti-pigmentation.Inthepresentstudy,recombinanthumantyrosinasewas usedasaprobetoassayitsinhibitoryactivityfromwaterextractsofCodiaeumvariega,Schefflera arboricola,IxorawilliamsiiandIxoraduffiicv.Amongthem,waterextractfromIxoraduffii cvexhibitedseriouslyinhibitoryactivityofrecombinanthumantyrosinase(ICS0of58.38mg/ ml)equivalenttoKojicacid(IC50of58.31mg/ml). Keyword:recombinanthumantyrosinase;inhibitors;waterextractfromIxoraduffiicv and 3一O—b—D—glucoside，b-sitosterolstigmasterol mixture)l D--,8，2口--．O 一7)一ent—epicatechin)I