2株酵母菌单倍体原生质体形成与再生条件的研究.pdf

2011No．11 China ·42· SerialNo．236 Brewing 2株酵母菌单倍体原生质体形成与再生条件的研究 易弋，赵东玲，黎娅，龚熠，黄翠姬，伍时华· (广西工学院生物与化学工程系，广西柳州545006) GJ2008单倍体原生质体的形成率和再生率均达到最大。形成率分别是98．14％和97．19％。再生率分别是17．29％fF915．78％． 关键词：酿酒酵母；酒精高产：原生质体；形成率；再生率 中图分类号：TS261．1 文献标识码：A 文章编号：0254—5071(2011)11-0042—04 and two cetev／siae Theformation high-alcohol-yield regenerationofprotoplast丘Dm Sacclmromyccshaploid刚rains ⅥYi，ZHAO Ya,GONGYi，HUANG Shihua* Donglin,LI cuiji。WU andChcmicM (OemnnentofBiologicM Engineering,G脚sxiUniversityofTechnology,Liuzhou545006,China) Ah嗽Theconditiomofformationand fromtwo cerevisiacstrainsGGFSl6 regenerationofprotoplasthigh—alcohol-yieldSaccharomyceshaploid and conditions culturedfor with at37℃for30rain GJ2008wem were勰follows：the 1．5％snailase optimized．Theoptimum haploid 8h,hydrolyzed after and 8UClOt把Wele these forma- used嬲osmoticsmbflizer．Underconditions．the pretreatmentbyO．2％mereaptoethanolO．1％EDTA-Na2；1709／L tionrate fromthe strainsreached ratereached GGFSl6andGJ2008 98．14％and 17．290／O ofprotoplast haploid 97．190／O，respectively．Theregeneration and 15．78％，respectively．． rate Keywords：Saccharomycescerevisiae=,,high-alcohol-yield；protoplast；formationrate；re