Development of Galactose Biosensor Based on Functionalized ZnO Nanorods with Galactose Oxidase.pdfVIP

Hindawi Publishing Corporation Journal of Sensors Volume 2012, Article ID 696247, 7 pages doi:10.1155/2012/696247 Research Article Development of Galactose Biosensor Based on Functionalized ZnO Nanorods with Galactose Oxidase K. Khun, Z. H. Ibupoto, O. Nur, and M. Willander Division of Physical Electronic and Nanotechnology, Department of Science and Technology, Linkoping University, ¨ Campus Norrkoping, 60174 Norrkoping, Sweden ¨ ¨ Correspondence should be addressed to K. Khun, kimleang.khun@liu.se Received 31 December 2011; Revised 28 April 2012; Accepted 13 May 2012 Academic Editor: P. Siciliano Copyright © 2012 K. Khun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The fabrication of galactose biosensor based on functionalised ZnO nanorods is described. The galactose biosensor was developed by immobilizing galactose oxidase on ZnO nanorods in conjunction with glutaraldehyde as a cross-linker molecule. The IRAS study provided evidence for the interaction of galactose oxidase with the surface of ZnO nanorods. The electromotive force (EMF) response of the galactose biosensor was measured by potentiometric method. We observed that the proposed biosensor has a linear detection range over a concentration range from 10 mM to 200 mM with good sensitivity of 89.10 ± 1.23 mV/decade. In addition, the proposed biosensor has shown fast time response of less than 10 s and a good selectivity towards galactose in the presence of common interferents such as ascorbic