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Early Gene Expression in Wounded Human Keratinocytes Revealed by DNA Microarray Analysis.pdf
Comparative and Functional Genomics
Comp Funct Genom 2003; 4: 47–55.
Published online in Wiley InterScience (). DOI: 10.1002/cfg.239
Conference Paper
Early gene expression in wounded human
keratinocytes revealed by DNA microarray
analysis
1 ` 1 2 1 3
Manal A. Dayem , Chimene Moreilhon , Laurent Turchi , Virginie Magnone , Richard Christen ,
Gilles Ponzio2 1
and Pascal Barbry *
1 ´
Laboratoire de Physiologie Genomique des Eucaryotes, CNRS/UNSA UMR 6097, IPMC, F-06560 Sophia Antipolis, France
2 ´ ´
Laboratoire de Biologie et Physiologie de la Peau, INSERM U385, Faculte de Medecine, F-06107 Nice, France
3Laboratoire de Physiologie des Membranes Cellulaires, CNRS UMR6078 Bat. J. Maetz, Villefranche-sur-Mer, France
*Correspondence to: Abstract
Pascal Barbry, Laboratoire de
´ Wound healing involves several steps: spreading of the cells, migration and prolifer-
Physiologie Genomique des
Eucaryotes, IPMC, 660, Route ation. We have profiled gene expression during the early events of wound healing in
des Lucioles, Sophia Antipolis, normal human keratinocytes with a home-made DNA microarray containing about
06560 Valbonne, France. 1000 relevant human probes. An original wounding machine was used, that allows the
E-mail: barbry@rs.fr wounding of up to 40% of the surface of a confluent monolayer of cultured cells grown
on a Petri dish (compared with 5% with a classical ‘scratch’ method). The two aims
of the present study were: (a) to validate a limited numb
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