Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy.pdfVIP

Hindawi Publishing Corporation Journal of Toxicology Volume 2011, Article ID 973172, 11 pages doi:10.1155/2011/973172 Research Article Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy Shinobu Ito,1, 2 Tomohisa Mori,3 Hideko Kanazawa,2 and Toshiko Sawaguchi4 1 I.T.O. Provitamin Research Center, 1-6-7-3F Nakamachi, Musashino, Tokyo, Japan 2 Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan 3 Department of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska at Omaha, Omaha, NE 68182, USA 4 Department of Occupational Therapy, Faculty of Regional Health Therapy, Teikyo Heisei University, 4-1 Uruido-minami, Ichihara, Chiba, Japan Correspondence should be addressed to Toshiko Sawaguchi, tsawagu@ Received 14 January 2011; Revised 29 March 2011; Accepted 12 April 2011 Academic Editor: Lucio Guido Costa Copyright © 2011 Shinobu Ito et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct s