Generation and Analysis of Expressed Sequence Tags from Olea europaea L.pdfVIP

Hindawi Publishing Corporation Comparative and Functional Genomics Volume 2010, Article ID 757512, 9 pages doi:10.1155/2010/757512 Research Article Generation and Analysis of Expressed Sequence Tags from Olea europaea L. Nehir Ozdemir Ozgenturk,1 Fatma Oruc¸,1 Ugur Sezerman,2 Alper Kuc¸ukural,2 Senay Vural Korkut,1 Feriha Toksoz,3 and Cemal Un3 1 Department of Biology, Faculty of Science and Arts, Yildiz Technical University, Davutpasa Street 124, 34210 Merter/Istanbul, Turkey 2 Faculty of Engineering and Natural Sciences, Sabanci University, 34956 Tuzla/Istanbul, Turkey 3 Department of Biology, Faculty of Science, Ege University, 35100 Bornova/Izmir, Turkey Correspondence should be addressed to Nehir Ozdemir Ozgenturk, nehirozdemir@ Received 10 August 2010; Accepted 13 October 2010 Academic Editor: Antoine Danchin Copyright © 2010 Nehir Ozdemir Ozgenturk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Olive (Olea europaea L.) is an important source of edible oil which was originated in Near-East region. In this study, two cDNA libraries were constructed from young olive leaves and immature olive fruits for generation of ESTs to discover the novel genes and search the function of unknown genes of olive. The randomly selected 3840 colonies were sequenced for EST collection from both libraries. Readable 2228 sequences for olive leaf and 1506 sequences for olive fruit were assembled into 205 and 69 contigs, respectively, whereas 2478 were singletons. Putative functions of all 2752 differentially expressed unique sequences were designated by gene homology based on BLAST and