Global Transcriptional Analysis of Yeast Cell Death Induced by Mutation of Sister Chromatid Cohesin.pdfVIP

Hindawi Publishing Corporation Comparative and Functional Genomics Volume 2008, Article ID 634283, 16 pages doi:10.1155/2008/634283 Research Article Global Transcriptional Analysis of Yeast Cell Death Induced by Mutation of Sister Chromatid Cohesin Qun Ren,1 Hui Yang,1 Bifeng Gao,2 and Zhaojie Zhang1 1 Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA 2 Microarray Core, University of Colorado Health Science Center, Denver, CO 80262, USA Correspondence should be addressed to Zhaojie Zhang, zzhang@ Received 23 December 2007; Revised 31 March 2008; Accepted 22 April 2008 Recommended by Brian Wigdahl Cohesin is a protein complex that regulates sister chromatid cohesin during cell division. Malfunction in chromatid cohesin results in chromosome missegregation and aneuploidy. Here, we report that mutations of MCD1 and PDS5, two major components of cohesin in budding yeast, cause apoptotic cell death, which is characterized by externalization of phosphatidylserine at cytoplasmic membrane, chromatin condensation and fragmentation, and ROS production. Microarray analysis suggests that the cell death caused by mutation of MCD1 or PDS5 is due to the internal stress response, contrasting to the environmental or external stress response induced by external stimuli, such as hydrogen peroxide. A common feature shared by the internal stress response and external stress response is the response to stimulus, including response to DNA damage, mitochondria functions, and oxidative stress, which play an important role in yeast apoptotic cell death. Copyright © 2008 Qun Ren et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium