Inflammatory and Angiogenic Protein Detection in the Human Vitreous Cytometric Bead Assay.pdfVIP

Hindawi Publishing Corporation Journal of Ophthalmology Volume 2011, Article ID 459251, 4 pages doi:10.1155/2011/459251 Clinical Study Inflammatory and Angiogenic Protein Detection in the Human Vitreous: Cytometric Bead Assay M. J. Koss, M. Pfister, and F. H. Koch Section of Vitreo-Retinal Surgery, Department of Ophthalmology, Hospital of the Goethe University, Goethe University, 60590 Frankfurt am Main, Germany Correspondence should be addressed to M. J. Koss, michael.koss@kgu.de Received 13 July 2011; Accepted 22 November 2011 Academic Editor: Andrew G. Lee Copyright © 2011 M. J. Koss et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Introduction. To evaluate clinical feasibility and reproducibility of cytometric bead assay (CBA) in nondiluted vitreous samples of patients with age-related macular degeneration (ARMD), diabetic macular edema (DME), and central retinal vein occlusion (CRVO). Methods. Twelve patients from a single clinics day qualified for intravitreal injections (ARMD n = 6, DME n = 3, CRVO n = 3) and underwent a combination treatment including a single-site 23 gauge core vitrectomy which yielded a volume of 0.6 mL undiluted vitreous per patient. Interleukin-6 (IL-6), vascular endothelial growth factor isoform A (VEGF-A), and monocyte chemo-attractant protein-1 (MCP-1) were assessed directly from 0.3 mL at the same day (fresh samples). To assess the ◦ reproducibility 0.3 ml were frozen for 60 days at −80 , on which the CBA was repeated (frozen samples). Results. In the fresh sam- ples