Mapping of fluorescence anisotropy in living cells by ratio imaging. Application to cytoplasmic viscosity.PDFVIP

Mapping of fluorescence anisotropy in living cells by ratio imaging Application to cytoplasmic viscosity James A. Dix and A. S. Verkman Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0532 ABSTRACT Steady-state and time-re- by-side gave values equivalent to r ond appearing at late times from the solved fluorescence properties of measured by cuvette fluorometry. Cy- interstitial region (r = 0.08 ± 0.03), probes incorporated into living cells t