阿维菌素生物合成与途径工程.pdfVIP

生物技术通报 BI OTECHN OL OG Y B ULLETI N 2003 6 苏建亚 沈晋良 ( , 210095) : 阿维菌素是一种高效安全的大环内酯杀虫杀螨剂本文介绍了阿维菌素 物合成的步骤及参与合 成步骤的有关酶系统和基因簇对阿维菌素8 个组分合成的遗传控制基因, 特别是对其中B 组分合成的遗传控 1a 制位点进行讨论分析, 并介绍了利用途径工程改造阿维链霉菌 产合成单 一高效组分B 和提高活性组分产量的 1a 研究进展 : 阿维菌素 途径工程 杀虫剂 基因工程 物合成 Biosynthesis and Pathway Engineering of Abamectin Su Jianya Shen Jinliang ( K ey L aboratory of Monitoring and Management of Plant Disease and Insects M inistry of Agr iculture Nanj ing Agricultur al University, Nanj ing 210095) Abstract: Abamectin is a highly effective and safety macr lide insecticide. This paper reviewed the pathw ay f abamectin bi synthesis, and the enzyme system and gene cluster inv lved in the bi synthesis. The genes that g vern the synt hesis f 8 abamectin c mp nents were analyzed, and especially genet ic c ntr lling sites f the c mp nent B synthesis 1a w ere discussed. The research pr gresses achieved in the pr ducti n f single highly effective c mp nent B1a and y ield in- crease f active c mp nents w ith the aid f pat hw ay engineering t m dify Strept myces avermitilis were reviewed. Key w ords: Abamectin Pathway engineering Insecticide Gene engineering Bi synthesis ( Pathw ay Engineering) ( trep tomyces aver- , DNA mitilis) , , ( 1, 20 , 1) , 2 DNA , 8 , 1. 8% 80% B , 20%