分子生物学基本技术定稿精要.pptVIP

* Serratia marcescens 粘质沙雷菌(S. marcescens) Mycobacterium phlei 草分枝杆菌(M. phlei) * DNA hybridization. Two DNA samples to be compared are completely denatured by heating. When the two solutions are mixed and slowly cooled, DNA strands of each sample associate with their normal complementary partner and anneal to form duplexes. If the two DNAs have significant sequence similarity, they also tend to form partial duplexes or hybrids with each other: the greater the sequence similarity between the two DNAs, the greater the number of hybrids formed. Hybrid formation can be measured in several ways.