乌珠穆沁羊生长程中肌纤维结构特性变化研究.pdfVIP

摘 要 肌肉的基本组成单位是肌纤维，肌纤维的组织学特性直接影响肌肉的品质。本论 文选用1 至18 月龄自然放牧条件下的乌珠穆沁羊为研究对象，利用组织学染色法， 即肌纤维结构HE 染色法和结缔组织EVG 染色法，观察随月龄增加肌纤维直径以及结 缔组织中肌内膜和肌束膜厚度的变化。利用ATPase 法区分骨骼肌中肌纤维类型并分 析其随月龄增加的变化规律。利用SDS分离肌球蛋白重锁(MyCH)异构体。结 果表明： 随月龄的增加骨骼肌中肌纤维直径均有所增大。其中背最长肌(LD)肌纤维直径增 大幅度最缓，整体上，在9 月龄时肌纤维直径总体达到最佳阶段。 骨骼肌肌内结缔组织中肌内膜厚度不断增厚，而肌束膜厚度在三角肌(DAO)和背 最长肌中随月龄的增加而不断增厚，而肱三头肌(TB)、半腱肌(ST)和股二头肌(BF) 中 肌束膜厚度在9～12 月龄之间下降，之后又开始上升。 整体上，乌珠穆沁羊骨骼肌中Ⅰ型、Ⅱa 型肌纤维(红肌)含量相对较高，其中LD 中绝大部分是红肌，后腿部位半腱肌(ST)在12 月龄和股二头肌(BF)在18 月龄时白肌 含量较高，影响肉质，不利于屠宰生产。 本研究对1 至18 月龄不同生长阶段乌珠穆沁羊的肌肉结缔组织以及肌纤维的变 化特征进行研究。旨在掌握随月龄的增加不同部位肌肉结缔组织以及肌纤维类型的变 化规律，为指导畜牧业生产和肉制品加工提供可靠的参考数据。 关键词：骨骼肌；肌内膜；肌束膜；肌纤维类型；肌球蛋白 Structural Characteristics Changes in the Skeletal Muscle Fibers During development of Wuzhumuqin Sheep Abstract Muscle fibers were the basic composition of skeletal muscle. The histology characteristics of muscle fibers directly influenced the muscle quality. The natural grazing Wuzhumuqin sheeps, from 1 to 18 mo. of age, were employed in the study. And used the histochemical methods, HE and EVG, to analysis the changes of intramuscular connective tissue during the growth of Wuzhumuqin sheep. The changes of the muscle fiber types were determined by method of ATPase. The method of SDSwas employed to separated myosin heavy chain isoforms (MyCH). There are main conclusions as follow: The diameter of skeletal muscle fibers were increasing during the growth of Wuzhumuqin sheep . The diameter of LD was increased slowly. In general, the diameter of skeletal muscle fibers achieved a best stage in 9 month-age. The thickness of endomysium was increasing during the growth of Wuzhumuqin sheep. The thickness of perimysium in DAO and LD were increasing wi