小麦胚芽粉吸收体外模拟及其营养物质的测定.pdfVIP

摘 要 本研究内容包括对小麦胚芽超微粉的体外模拟环境中对消化吸收率的测定以及 利用原子吸收分光光度计和氨基酸自动分析仪对其微量元素和氨基酸含量进行测定。 本研究中使用的小麦胚芽超微粉是由内蒙古某食品有限公司采用国际上先进的超微 粉碎技术生产的500 目 （直径25μm ）左右的超微粉，研究人体对其消化吸收效果和 其中的微量元素及氨基酸的含量。试验中，模拟了人体消化道内环境的温度、pH 等 条件，采用胃-胰蛋白酶两步法对小麦胚芽超微粉进行体外消化，并用超滤膜模拟小 肠内膜过滤小麦胚芽粉测定的其消化吸收率在50%左右。利用原子吸收分光光度计测 得的小麦胚芽超微粉中微量元素钙、锌、铁含量分别为：23.76mg/100g 、12.66mg/100g、 72.82mg/100g 。利用氨基酸自动分析仪测定小麦胚芽超微粉中的氨基酸的总含量 24.559 mg/100g 。通过测定结果可以得出：人体对小麦胚芽超微粉能够很好的消化吸 收，并且钙、锌、铁三种人体必需的微量元素及各种氨基酸的含量比牛奶和鸡蛋等普 通的营养品高出3～4 倍。因此，小麦胚芽超微粉可以作为营养保健品进行开发利用。 关键词：小麦胚芽超微粉；吸收率；微量元素；氨基酸 Detemination of wheat germ power absorption rate invitro and its nutrients Abstract This contents of this study include the digestion absorption rate determination of ultrafine powders of wheat germ in vitro environment and the use of atomic absorption spectrometer and amino acid automatic analyzer to measure its trace elements and amino acid levels .Ultrafine powder of wheat germ used in this study was from a food company in Inner Mongolia, which using advanced ultrafine grinding technology to produce 500 （diameter 0.25μm）of ultrafine study of human digestion and absorption effects, one of the trace elements and amino acids the content test to simulate the environment inside the human digestive tract, such as temperature, pH conditions, using the stomach to a two-step method-trypsin in vitro digestibility of wheat germ ultrafine and simulated small intestine intima ultrafitration membrane filtration wheat germ powder determination of digestion and absorption rate of about 50%. Using atomic absorption spectrophotometer to measure the trace elements in wheat germ ultrafine, such as calcium, zinc, iron, conrtent, which is 23.76mg/100g; 12.66 mg/100g, 72.82 mg/100g, respectively. Resu