温室嫁接黄瓜对温、高co2浓度的响应机理.pdfVIP

摘 要 采用黑籽南瓜砧木和“津春四号”黄瓜品种嫁接苗为试材，通过高温、高 CO2 浓 度对温室嫁接黄瓜生长发育、光合特性、光合产物、Rubisco 羧化酶活性、酶防御 系统以及嫁接黄瓜产量和品质的研究，得出以下结论： (1)温室嫁接黄瓜通过高温、高 CO2 浓度处理，使光合有效辐射、胞间 CO2 浓度、 气孔导度和叶温提高，蒸腾速率降低，叶绿素含量升高，淀粉合成增加，淀粉转换 成糖类增加，从而使温室嫁接黄瓜的植株高度增加，进行光合作用的叶面积和叶片 厚度增加。（2 ）温室嫁接黄瓜通过高温、高 CO2 浓度处理，使 Rubisco 羧化酶活性 提高，从而提高对 CO2 的固定；嫁接黄瓜的质膜透性、Pro 、MDA 含量均低于正常 管理，酶防御系统（SOD、POD 、CAT ）活性均高于正常管理。有效地减弱了高温 对嫁接黄瓜幼苗叶片引起的伤害，提高了嫁接黄瓜植株防御高温的能力，对温室嫁 接黄瓜耐热性具有促进作用。从以上两方面表明：高温、高 CO2 浓度最终减少了温 室嫁接黄瓜的光合“午休”现象，提高温室嫁接黄瓜的产量和品质。 关键词：嫁接黄瓜；高温；高 CO2 浓度；酶；产量 High Temperature and High CO2 Concentration Response Mechanism of Grafting Cucumber in Greenhouse Abstract By using the seed pumpkin root stock and tianjin spring 4 cucumber varieties JiaJieMiao as test materials, through the high temperature, high concentration of CO2 greenhouse grafting cucumber growth, photosynthetic characteristics, photosynthesis product, Rubisco carboxylase (Rubisco) activity, enzyme defense system and grafting yield and quality of cucumber, draw the following conclusion: (1) the cucumber greenhouse grafting through high temperature and increased CO2 concentration; processing, make the photosynthetic available radiation, the intercellular CO2 concentrations, and stomatal conductance and YeWen improve, transpiration rate, chlorophyll content increases, starch synthesis increase, starch convert sugar increase, to make the cucumber greenhouse grafting the increase plant height, leaf area and photosynthesis of leaf thickness increase. (2) greenhouse grafting through high temperature and the cucumber was CO2 concentration processing, make the Rubisco carboxylase (Rubisco) activity imp