腐胺提高黄瓜根际低氧耐性的生理机理.pdfVIP

中国农业科学 2009,42(5):1854-1858 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.05.045 腐胺提高黄瓜根际低氧耐性的生理机理 师 恺，顾 敏，于海静，姜玉萍，周艳虹，喻景权 （浙江大学园艺系/农业部园艺植物生长发育与生物技术重点开放实验室，杭州 310029 ） 摘要：【目的】明确腐胺（putrescine，Put）对黄瓜根际低氧耐性的作用及其机制。【方法】以黄瓜为材料， 分别在抑制和不抑制酸还原过程的条件下，在水培体系中对植物进行Put和根际低氧胁迫处理。【结果】外源Put 显著提高了硝酸还原酶的实际（NR ）和最大（NR ）活性，且这种作用在低氧胁迫植株中表现尤为明显。Put缓 act max 解了低氧胁迫下黄瓜叶片的净光合速率（Pn）、气孔导度（Gs）、胞间CO2 浓度（Ci）以及根系线粒体总呼吸（Vt）、 细胞色素途径呼吸（Vcyt）和交替途径呼吸（Valt）速率的下调。然而，当硝酸还原过程被钨酸钠抑制后，Put 不仅失去了对光合作用及线粒体呼吸的缓解作用，反而进一步加剧了这两个基础代谢过程活性的下降。【结论】Put 参与提高黄瓜对低氧胁迫抗性的作用和硝酸还原过程有关。 关键词：黄瓜；腐胺；光合作用；线粒体呼吸；硝酸还原；硝酸还原酶 Physiological Mechanism of Putrescine Enhancement of Root-Zone Hypoxia Tolerance in Cucumber Plants SHI Kai, GU Min, YU Hai-jing, JIANG Yu-ping, ZHOU Yan-hong, YU Jing-quan 1 ( Department of Horticulture, Zhejiang University/Key Laboratory of Horticultural Plant Growth, Development and Biotechnology, Agricultural Ministry of China, Hangzhou 310029) Abstract: 【Objective 】 The purpose of this study is to investigate the physiological mechanism involved in the enhancement effects of putrescine (Put) on hypoxic stress tolerance. 【Method 】 Cucumber (Cucumis sativus L.) seedlings were grown hydroponically and subjected to Put and root-zone hypoxia treatment with or without inhibition the internal nitrate reduction reaction. 【Result 】 The actual (NRact) and maximal (NRmax) nitrate reductase activities were greatly enhanced by Put application, particularly in roots exposed to hypoxia. The reduction of leaf net CO2 assimilation rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci)