广西地不容的杀虫活性及有效成分研究.pdfVIP

中国农业科学: 2005,38(3):523-527 Scientia Agricultura Sinica 广西地不容的杀虫活性及有效成分研究 邓业成 1，2，徐汉虹2 1 2 （广西师范大学生命科学学院, 桂林 541004; 华南农业大学昆虫毒理研究室/农药与化学生物学教育部重点实验室, 广州 510642 ） 摘要：首次研究了广西地不容的杀虫活性及有效成分。结果表明，广西地不容植株各部位对褐飞虱均有触杀 活性，而以块根甲醇提取物的活性最高，LD50 值为 1.5794 µg/头。通过活性跟踪，从块根提取物中分离鉴定出主 要杀虫有效成分 l-罗默碱，其对褐飞虱具有很高的触杀毒力，LD 值为0.0443 µg/头，是防治褐飞虱的化学杀虫 50 剂马拉硫磷毒力的7.48倍。l-罗默碱对褐飞虱也有胃毒杀虫作用。 关键词：广西地不容；杀虫活性；褐飞虱； l-罗默碱 Studies on Insecticidal Activities and Active Ingredients of Stephania kwangsiensis Lo. DENG Ye-cheng1,2 , XU Han-hong2 1 2 （College of Life Science, Guangxi Normal University, Guilin 541004; Laboratory of Insect Toxicology , South China Agricultural University/ Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642 ） Abstract: Insecticidal activities and active ingredients of Stephania kwangsiensis were studied for the first time. The results showed that all parts of Stephania kwangsiensis had contact activity to brown planthopper, Nilaparvata lugens, and the contact activity of methanol extract from tuber was highest, with a LD50 value being 1.5794 µg/female. l-roemerine was isolated from tuber of Stephania kwangsiensis and identified, and it was the main active ingredient. l-roemerine had high contact toxicity to brown planthopper, with a LD50 value being 0.0443 µg/female. Contact toxicity of l-roemerine to brown planthopper was 7.48 times that of malathion, the convientional chemical insecticide used for controlling brown planthopper. l-roemerine had also stomach po