海岛棉DNA导入陆地棉栽培品种获得变异种质的初步遗传分析.pdfVIP

中国农业科学 2005,38(8):1528-1533 Scientia Agricultura Sinica 海岛棉DNA导入陆地棉栽培品种获得变异种质的初步遗传分析 王芙蓉, 张 军, 刘任重, 刘勤红, 张传云, 刘国栋 ( 山东省农业科学院棉花研究中心，济南 250100 ) 摘要：利用花粉管通道导入技术将海岛棉（Gossypium barbadense L.）品种海7124 的基因组总DNA导入陆 地棉（G. hirsutum L.）栽培品种石远 321 中，获得了纤维品质优良的新种质系，并且在导入后代中发现了 1 个 形态性状发生变异的突变体。对突变体进一步的遗传分析表明，突变性状可能受 1 个纯合致死的显性基因控制， 利用 SSR 对变异材料进行研究发现，外源 DNA 已经整合在陆地棉的基因组中，并推测某些 DNA 片段可能是通过同 源重组的机制整合到陆地棉染色体上的。 关键词：海岛棉；陆地棉；花粉管通道导入；种质；遗传分析；SSR Genetic Analysis of Upland Cotton Germplasm Obtained from Introduced DNA from Island Cotton WANG Fu-rong, ZHANG Jun, LIU Ren-zhong, LIU Qin-hong, ZHANG Chuan-yun, LIU Guo-dong (Cotton Reseach Center, Shandong Academy of Agricultural Science, Jinan 250100) Abstract: The genomic DNA of island cotton (Gossypium barbadense L. cv. Hai7124) was introduced into the upland cotton (G. hirsutum L. cv. Shiyuan321) by pollen tube pathway technique. New germplasm of high quality of fiber and a mutant with obviously change of morphology were obtained. Genetic analysis of the mutant indicated that the phenotype of the mutant was possibly controlled by a dominant allele that has homologous lethal effect. The result of SSR (simple sequence repeats) analysis showed that at least some exogenous DNA fragments were integrated into the genome of upland cotton Shiyuan321. It is deduced that some of the DNA fragments were integrated by homologous recombination mechanism. Key words: Gossypium barbadense L.; Gossypium hirsutum L.; Pollen tube pathway; Germplasm; Genetic analysis; SSR 作物种质资源(crop germplasm)，包括作物各类品 异种质。该方法是周光宇在 20 世纪 80 年代建立的， 种、品系、类型、野生种和近缘植物，是改良农作物 其基本原理是 DNA 片段杂交，由于该技术克服了远 的基因来源[1] 。但是受生殖隔离的限制，植物野生种 缘杂交中的生殖隔离，因而在利用远缘种质拓展栽培