河北邢台和江苏南京地区棉蚜对丁硫克百威和吡虫啉的抗性.pdfVIP

中国农业科学 2013,46(16):3377-3383 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.16.008 河北邢台和江苏南京地区棉蚜对丁硫克百威和吡虫啉的抗性 王 娜，郎志飞，贺 康，李 飞 （南京农业大学植物保护学院，南京210095 ） 摘要：【目的】对河北邢台和江苏南京地区棉蚜（Aphis gossypii Glover）进行抗性监测，了解棉蚜对丁硫 克百威和吡虫啉的抗性发展情况，为抗性治理提供理论依据。 【方法】以用药水平最低的新疆南疆种群作为相对 敏感种群，通过室内毒力测定、离体酶活力测定、相对表达量分析，研究新疆南疆、河北邢台及江苏南京3 个地 区棉蚜对丁硫克百威和吡虫啉潜在的代谢抗性机制。 【结果】河北邢台和江苏南京地区的棉蚜对丁硫克百威仍处 于敏感阶段；但对吡虫啉的抗性已有所发展，抗性水平分别为4.87 和8.72 倍，为低水平抗性阶段。解毒代谢酶 活力对比研究发现，3 个地区田间种群之间多功能氧化酶、酯酶及谷胱甘肽转移酶的活力均存在显著差异，江苏 南京种群的酯酶活力显著高于新疆南疆种群。定量PCR 分析表明，河北邢台地区棉蚜的羧酸酯酶表达量较高，是 新疆种群棉蚜的3.28 倍。【结论】田间棉蚜已对吡虫啉产生了低水平的抗性，酯酶活力升高在棉蚜对吡虫啉低水 平抗性形成中发挥作用。 关键词：棉蚜；丁硫克百威；吡虫啉；抗性 Resistance of Field Population Aphis gossypii Glover to Carbosulfan and Imidacloprid in Xingtai and Nanjing District WANG Na, LANG Zhi-fei, HE Kang, LI Fei (College of Plant Protection, Nanjing Agricultural University, Nanjing 210095) Abstract: 【Objective 】 The objective of this study is to monitor the insecticide resistance of Aphis gossypii Glover in filed to carbosulfan and imidacloprid, and to provide a better understanding of resistance mechanism. 【Method 】The cotton aphids were collected from fields in Nanjiang city of Xinjiang Uygur Autonomous Region (XJ), Xingtai city of Hebei Province (HB), and Nanjing city of Jiangsu Province (JS). The resistances of field cotton aphid to conventionally used insecticides imidacloprid and carbosulfan were investigated. The cotton aphids from Xinjiang were treated as the sensitive strain. The bioassay, enzyme activities and mRNA relative expression levels by qPCR were performed to study the resistance ratios and their potential molecular