宁强矮马线粒体DNA D-loop区的遗传多样性.pdfVIP

中国农业科学 2012,45(8): 1587-1594 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.08.014 宁强矮马线粒体DNA D- loop区的遗传多样性 张 涛, 路宏朝 （陕西理工学院生物科学与工程学院，陕西汉中 723000 ） 摘要：【目的】研究宁强矮马线粒体DNA （mitochondrial DNA，mtDNA）D-loop 区的遗传多样性及其母系起 源进化，为其遗传资源保护提供理论数据。【方法】采用PCR 和测序技术分析 12 匹宁强矮马mtDNA D-loop 区的 600 bp 核酸序列，并基于mtDNA D-loop 区序列将宁强矮马和NCBI 公布的其它马种进行系统树构建。【结果】 宁强矮马群体存在9 种单倍型，检测到34 处SNP 位点，占所分析位点总数的5.67%，各单倍型间的遗传距离为 0.002—0.035，单倍型多样性为0.978±0.054，核苷酸多性为0.01988±0.00818。聚类分析表明宁强矮马分布在 4 个支系中，与胡克尔马、设特兰马、德保马、蒙古马和车巨马亲缘关系较近，与纯血马、云南马和韩国济州岛本土 马的亲缘关系较远。【结论】宁强矮马具有比较丰富的遗传多样性，在进化的历史过程中受到其它马种的影响较大。 关键词：宁强矮马; mtDNA D-loop; 遗传多样性; 母系起源 Genetic Diversity of Mitochondrial DNA D-loop Sequences in Ningqiang Pony ZHANG Tao, LU Hong-zhao (School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, Shaanxi) Abstract: 【Objective 】In order to provide a scientific and theoretical basis for genetic resource protection of Ningqiang pony, the genetic diversity of mitochondrial DNA (mtDNA) D-loop and maternal origin of Ningqiang pony was investigated. 【Method 】 Twelve 600 bp mtDNA D-loop sequences were analyzed by PCR and sequencing techniques, and the phylogenetic trees were constructed based on the mtDNA D-loop sequences of Ningqiang pony samples and other equine D-loop sequence available from NCBI. 【Result 】There were 9 unique haplotypes with 34 polymorphic sites in Ningqiang pony, which account for 5.67% of 600 bp sequence analyzed. The haplotype diversity (h) and nucleotide diversity (π) were 0.978±0.054 and 0.01988±0.00818, respectively ．Moreover, Ningqiang pony distributed in four branches, and are closely related to H