禽源奇异变形杆菌超广谱beta;-内酰胺酶基因的分子特征.pdfVIP

中国农业科学 2013,46(7):1463-1469 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.07.017 禽源奇异变形杆菌超广谱β-内酰胺酶基因的分子特征 潘玉善，苑 丽，吴 华，刘建华，刘珍珍，赵宜双，胡功政 （河南农业大学牧医工程学院，郑州450002 ） 摘要：【目的】研究禽源奇异变形杆菌携带超广谱β-内胺酰酶基因的亚型和blaCTX-M 基因的上下游环境。【方 法】对分离、鉴定的奇异变形杆菌进行药物敏感性试验、ESBLs 产酶确证试验、耐药基因检测和接合试验；利用 PCR 定位技术（PCR mapping）分析blaCTX-M 的基因环境。【结果】ESBLs 确证试验表明，21 株禽源奇异变形杆菌有 10 株是阳性表型。PCR 扩增和测序结果表明，最常见的ESBL 基因是blaCTX-M-14 (n=6) ,blaOXA-1 (n=6), 其次是blaCTX-M-65 (n=4)，同时也检测到了bla (n=1)，没有检测到bla 。序列分析表明，禽源奇异变形杆菌携带bla 的上游 OXA-10 SHV CTX-M 普遍存在ISEcp1B，下游均为IS903D。【结论】首次在禽源奇异变形杆菌中检测到了blaCTX-M-65 ，CTX-M 型超广谱β- 内酰胺酶基因在禽源奇异变形杆菌中已不在少见。 关键词：奇异变形杆菌；ESBLs；blaCTX-M ；插入序列元件；家禽 Molecular Characteristics of Extended-Spectrum β-Lactamases in Clinical Isolates of Proteus mirabilis from Poultry PAN Yu-shan, YUAN Li, WU Hua, LIU Jian-hua, LIU Zhen-zhen, ZHAO Yi-shuang, HU Gong-zheng (College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002) Abstract: 【Objective 】This experiment was conducted to explore the genotpye of extended-spectrum β-lactamases (ESBLs) gene and the genetic environment of blaCTX-M carried by Proteus mirabilis . 【Method 】The isolates were characterized by isolation and identification, antimicrobial susceptibility testing, confirmation of ESBLs, resistant genes screening, and conjugation experiments. PCR mapping was performed to investigate the genetic environment of blaCTX-M. 【Result 】Among the 21 P. mirabilis clinical isolates from poultry, 10 (47.6%) isolates exhibited extended-spectrum β-lactamase (ESBL) phenotypes. The most common types of ESBL gene screened by PCR